Kallikrein 13 Kit ELISA

N° du produit ABIN6719902

Détail du produit Kallikrein 13 Kit ELISA

Antigène: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: DKFZp586J1923, Kalikrein, Kallikrein 13, Kallikrein L4, Kallikrein Like 4, Kallikrein like 4 variant included, Kallikrein like gene 4, Kallikrein like protein 4, Kallikrein related peptidase 13, Kallikrein-13, Kallikrein-like protein 4, KLK-L4, KLK13, KLK13_HUMAN, KLKL 4, KLKL 4, KLKL4

Background: Kallikrein-13 is a protein that in humans is encoded by the KLK13 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. By genomic sequence analysis, KLK13 gene is mapped in a 300-kb region on chromosome 19q13.3-q13.4. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components. However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies.

Gene Name: KLK13

Production: Natural and recombinant human KLK13. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: G17-Q277

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK13 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Autre désignation: KLK13 (KLK13 Produits)

Synonymes: KLK-L4 Kit ELISA, KLKL4 Kit ELISA, Egfbp-2 Kit ELISA, mGk-13 Kit ELISA, kallikrein related peptidase 13 Kit ELISA, kallikrein related-peptidase 13 Kit ELISA, KLK13 Kit ELISA, Klk13 Kit ELISA

ID gène: 26085

NCBI Accession: NP_056411

UniProt: Q9UKR3

Pathways: Système du Complément