TNFRSF8 Kit ELISA

N° du produit ABIN6719910

Détail du produit TNFRSF8 Kit ELISA

Antigène: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD 30, CD153, CD30, CD30 antigen, CD30L receptor, Cytokine receptor CD30, D1S166E, KI 1, Ki 1 antigen, Ki-1 antigen, KI1, Lymphocyte activation antigen CD30, TNFRSF 8, TNFRSF8, TNFRSF8, TNR8_HUMAN, Tumor Necrosis Factor Receptor Superfamily Member 8, Tumor Necrosis Factor Receptor Superfamily Member 8

Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.

Gene Name: TNFRSF8

Production: Natural and recombinant human CD30.There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: F19-K379

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human CD30 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each human CD30 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Autre désignation: CD30 - TNFRSF8 (TNFRSF8 Produits)

Synonymes: CD30 Kit ELISA, D1S166E Kit ELISA, Ki-1 Kit ELISA, Cd30 Kit ELISA, Ki Kit ELISA, TNFRSF8 Kit ELISA, TNF receptor superfamily member 8 Kit ELISA, tumor necrosis factor receptor superfamily, member 8 Kit ELISA, TNFRSF8 Kit ELISA, Tnfrsf8 Kit ELISA

ID gène: 943

NCBI Accession: NP_001234

UniProt: P28908