Cadherin 5 Kit ELISA

N° du produit ABIN6719922

Détail du produit Cadherin 5 Kit ELISA

Antigène: Cadherin 5 (CDH5)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: 7B 4, 7B4, 7B4 antigen, CADH5_HUMAN, Cadherin 5, Cadherin 5 type 2, Cadherin 5, type 2 (vascular endothelium), Cadherin 5, type 2, VE cadherin (vascular epithelium), cadherin vascular endothelial, cadherin vascular endothelial 1, Cadherin-5, Cadherin5, CD 144, CD144, CD144 antigen, CDH 5, CDH5, CDH5 protein, Endothelial specific cadherin, FLJ17376, OTTHUMP00000174777, Vascular endothelial cadherin, Vascular epithelium cadherin, VE Cad, VE-cadherin, VEC

Background: Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.

Gene Name: CDH5

Production: Natural and recombinant mouse VE-Cad. There is no detectable cross-reactivity with other relevant proteins.herin

Standard: Expression system for standard: NSO, Immunogen sequence: D46-Q599

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse VE-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse VE-Cadherin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Cadherin 5 (CDH5)

Autre désignation: VE-Cadherin - CD144 (CDH5 Produits)

Synonymes: 7B4 Kit ELISA, AA408225 Kit ELISA, Cd144 Kit ELISA, VE-Cad Kit ELISA, VECD Kit ELISA, VEcad Kit ELISA, Vec Kit ELISA, wu:fc88a07 Kit ELISA, CD144 Kit ELISA, cadherin-5 Kit ELISA, cadherin 5 Kit ELISA, CDH5 Kit ELISA, Cdh5 Kit ELISA, cdh5 Kit ELISA

ID gène: 12562

NCBI Accession: NP_033998

UniProt: P55284

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2