Fetuin A Kit ELISA

N° du produit ABIN6719928

Détail du produit Fetuin A Kit ELISA

Antigène: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: A2HS, AHS, Ahsg, Alpha 2 HS glycoprotein, Alpha-2-HS-glycoprotein chain B, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, FETUA, FETUA_HUMAN, Fetuin-A, HSGA

Background: Alpha2-HS glycoprotein(AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein(AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

Gene Name: AHSG

Production: Natural and recombinant human Fetuin A. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A19-V367

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Fetuin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human Fetuin A standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Autre désignation: Fetuin A (AHSG Produits)

Synonymes: ahs Kit ELISA, a2hs Kit ELISA, hsga Kit ELISA, fetua Kit ELISA, wu:fb63g02 Kit ELISA, zgc:103687 Kit ELISA, AHSG Kit ELISA, MGC116429 Kit ELISA, A2HS Kit ELISA, AHS Kit ELISA, FETUA Kit ELISA, HSGA Kit ELISA, Aa2-066 Kit ELISA, pp63 Kit ELISA, alpha-2-HS-glycoprotein Kit ELISA, alpha 2-HS glycoprotein Kit ELISA, alpha-2-HS-glycoprotein 1 Kit ELISA, alpha-2-HS-glycoprotein L homeolog Kit ELISA, Cphamn1_1981 Kit ELISA, AHSG Kit ELISA, ahsg Kit ELISA, ahsg1 Kit ELISA, ahsg.L Kit ELISA, Ahsg Kit ELISA

ID gène: 197

NCBI Accession: NP_001613

UniProt: P02765