CXCL2 Kit ELISA

N° du produit ABIN6719957

Détail du produit CXCL2 Kit ELISA

Antigène: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: C-X-C motif chemokine 2, Chemokine (C-X-C motif) ligand 2, CINC-2a, Cxcl2, CXCL2_HUMAN, Gro beta, Gro-beta, GRO-beta(5-73), GRO-beta-T, GRO2, GRO2 oncogene, GROb, Growth-regulated protein beta, Hematopoietic synergistic factor, HSF, Macrophage inflammatory protein 2-alpha, Melanoma growth stimulatory activity beta, MGSA beta, MGSA-b, MIP 2, MIP 2a, MIP2, MIP2-alpha, MIP2A, ,SB-251353, SCYB2

Background: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein(MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein(MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water(aquaporins), glycerol and other small molecules in microbial, plant and animal cells. The standard used in this kit is recombinant mouse MIP-2(A28-N100), consisting of 73 amino acids with the molecular mass of 8KDa.

Gene Name: CXCL2

Production: Natural and recombinant mouse MIP-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence:  A28-N100

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse MIP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse MIP-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Other Performance Data: DOE: 2-8° C - 10/02/18 -20° C - 04/02/19

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Autre désignation: MIP-2 (CXCL2 Produits)

Synonymes: CINC-2a Kit ELISA, GRO2 Kit ELISA, GROb Kit ELISA, MGSA-b Kit ELISA, MIP-2a Kit ELISA, MIP2 Kit ELISA, MIP2A Kit ELISA, SCYB2 Kit ELISA, GRO3 Kit ELISA, Gro2 Kit ELISA, MIP-2 Kit ELISA, Mgsa-b Kit ELISA, Mip2 Kit ELISA, Scyb Kit ELISA, Scyb2 Kit ELISA, Mip-2 Kit ELISA, GROB Kit ELISA, MIP-2alpha Kit ELISA, C-X-C motif chemokine ligand 2 Kit ELISA, chemokine (C-X-C motif) ligand 2 Kit ELISA, CXCL2 Kit ELISA, Cxcl2 Kit ELISA

ID gène: 20310

NCBI Accession: NP_033166

UniProt: P10889

Pathways: Cellular Response to Molecule of Bacterial Origin