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sL-Selectin Kit ELISA

Rockland koa0299 sL-Selectin Reactivité: Humain Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
N° du produit ABIN6719972
N° du produit (Fournisseur): koa0299
  • Antigène Tous les produits sL-Selectin
    sL-Selectin (Selectin L (Soluble) (sL-Selectin))
    Reactivité
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5 pg/mL - 4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    N° du produit (Fournisseur)
    koa0299
    Fournisseur
    Rockland
    Fonction
    Human sL-Selectin Sandwich ELISA Kit for Quantitative Detection
    Marque
    AccuSignal™
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificité

    Production: Natural and recombinant human sL-Selectin. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité
    < 5 pg/mL
    Ingrédients
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Indications d'application

    ELISA_Dilution: 62.5pg/mL-4000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human sL-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human sL-Selectin standard solution and each sample be measured in duplicate.

    Commentaires

    Standard: Expression system for standard: NSO, Immunogen sequence: W39-N332

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Date de péremption
    12 months
  • Antigène Tous les produits sL-Selectin
    sL-Selectin (Selectin L (Soluble) (sL-Selectin))
    Autre désignation
    sL-Selectin (sL-Selectin Produits)
    Sujet

    Synonyms: CD62 antigen ligand, CD62L antigen, IgA nephropathy, L Selectin, L selectin precursor, LAM1, LECAM1, Leu8, Leukocyte adhesion molecule 1, Leukocyte endothelial cell adhesion molecule 1, Leukocyte surface antigen Leu8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LSEL, Ly-22, Ly-m22, Lyam 1, LYAM1, Lymph node homing receptor, Lymphocyte adhesion molecule 1, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, PLNHR, Selectin L, Selectin, lymphocyte

    Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

    ID gène
    6402
    NCBI Accession
    NP_000646
    UniProt
    P14151
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