Survivin Kit ELISA

N° du produit ABIN6719973

Détail du produit Survivin Kit ELISA

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, Baculoviral IAP repeat containing 5, Baculoviral IAP repeat-containing protein 5, BIRC5, BIRC5_HUMAN, EPR 1

Background: Survivin(also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50 % of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with the molecular mass of 34 KDa.

Gene Name: BIRC5

Production: Natural and recombinant human Survivin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: M1-D142

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Survivin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Survivin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Autre désignation: Survivin (BIRC5 Produits)

Synonymes: AP14 Kit ELISA, API4 Kit ELISA, EPR-1 Kit ELISA, AAC-11 Kit ELISA, Api4 Kit ELISA, TIAP Kit ELISA, survivin40 Kit ELISA, bir5a Kit ELISA, survivin Kit ELISA, BIR1 Kit ELISA, Su2 Kit ELISA, XBIR1 Kit ELISA, XSurvivin1 Kit ELISA, Xsvv1 Kit ELISA, api4 Kit ELISA, bir1-A Kit ELISA, epr-1 Kit ELISA, svv1 Kit ELISA, xSurvivin Kit ELISA, xsurv Kit ELISA, Su1 Kit ELISA, birc5.2 Kit ELISA, svv2 Kit ELISA, xSurvivin2 Kit ELISA, bir5b Kit ELISA, SIX Kit ELISA, birc5.2-a Kit ELISA, birc5.2-b Kit ELISA, baculoviral IAP repeat containing 5 Kit ELISA, baculoviral IAP repeat-containing 5 Kit ELISA, baculoviral IAP repeat containing 5a Kit ELISA, baculoviral IAP repeat-containing 5, gene 1 L homeolog Kit ELISA, baculoviral IAP repeat containing 5 L homeolog Kit ELISA, baculoviral IAP repeat containing 5b Kit ELISA, baculoviral IAP repeat containing 5 S homeolog Kit ELISA, BIRC5 Kit ELISA, Birc5 Kit ELISA, birc5a Kit ELISA, birc5.1.L Kit ELISA, birc5.2.L Kit ELISA, birc5b Kit ELISA, birc5.2.S Kit ELISA

ID gène: 332

NCBI Accession: NP_001012270

UniProt: O15392

Pathways: Apoptose, Cycle Cellulaire, Nuclear Hormone Receptor Binding