Survivin Kit ELISA

N° du produit ABIN6719973

N° du produit (Fournisseur): koa0369

Détail du produit Survivin Kit ELISA

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

N° du produit (Fournisseur): koa0369

Fournisseur: Rockland

Fonction: Human Survivin Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human Survivin. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 2 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Survivin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Survivin standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: M1-D142

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Autre désignation: Survivin (BIRC5 Produits)

Sujet:

Synonyms: API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, Baculoviral IAP repeat containing 5, Baculoviral IAP repeat-containing protein 5, BIRC5, BIRC5_HUMAN, EPR 1

Background: Survivin(also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50 % of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with the molecular mass of 34 KDa.

ID gène: 332

NCBI Accession: NP_001012270

UniProt: O15392

Pathways: Apoptose, Cycle Cellulaire, Nuclear Hormone Receptor Binding