TGFBI Kit ELISA

N° du produit ABIN6719979

Détail du produit TGFBI Kit ELISA

Antigène: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Beta ig-h3,b eta-IG-H3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato-epithelin, LCD1, RGD-CAP, RGD-containing collagen-associated protein, TGFBI, Transforming growth factor-beta-induced protein ig-h3

Background: Transforming growth factor, beta-induced, 68 kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Gene Name: TGFBI

Production: Natural and recombinant mouse beta IG-H3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: G24-H683

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse βIG-H3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse βIG-H3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Autre désignation: beta IG-H3 - TGFBI (TGFBI Produits)

Synonymes: TGFBI Kit ELISA, BIGH3 Kit ELISA, CDB1 Kit ELISA, CDG2 Kit ELISA, CDGG1 Kit ELISA, CSD Kit ELISA, CSD1 Kit ELISA, CSD2 Kit ELISA, CSD3 Kit ELISA, EBMD Kit ELISA, LCD1 Kit ELISA, 68kDa Kit ELISA, AI181842 Kit ELISA, AI747162 Kit ELISA, Beta-ig Kit ELISA, big-h3 Kit ELISA, Big-h3 Kit ELISA, MP70 Kit ELISA, MP78 Kit ELISA, hm:zeh0933 Kit ELISA, wu:fa13f05 Kit ELISA, zgc:110495 Kit ELISA, transforming growth factor beta induced Kit ELISA, transforming growth factor, beta induced Kit ELISA, transforming growth factor, beta-induced Kit ELISA, TGFBI Kit ELISA, Tgfbi Kit ELISA, tgfbi Kit ELISA

ID gène: 21810

NCBI Accession: NP_033395

UniProt: P82198