CCL3 Kit ELISA

N° du produit ABIN6720000

Détail du produit CCL3 Kit ELISA

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: C C motif chemokine 3, CCL3, CCL3_HUMAN, Chemokine ligand 3, Chemokine ligand 3, G0/G1 switch regulatory protein 19 1, G0/G1 switch regulatory protein 19-1, G0S19 1, Heparin binding chemotaxis protein, LD78-alpha, Macrophage inflammatory protein 1 alpha, Macrophage inflammatory protein 1-alpha, MIP 1 alpha, MIP-1-alpha, MIP-1-alpha, MIP1A, PAT 464.1, SCYA 3, SCYA3, SIS alpha, SIS beta, SIS-beta, Small inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Background: Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5 kDa.

Gene Name: CCL3

Production: Natural and recombinant human MIP-1 alpha. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence:  A27-A92

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIP-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human MIP-1 alpha standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Autre désignation: MIP-1 alpha (CCL3 Produits)

Synonymes: MIP-1alpha Kit ELISA, G0S19-1 Kit ELISA, LD78ALPHA Kit ELISA, MIP-1-alpha Kit ELISA, MIP1A Kit ELISA, SCYA3 Kit ELISA, MIP-1a Kit ELISA, Scya3 Kit ELISA, AI323804 Kit ELISA, LD78alpha Kit ELISA, MIP1-(a) Kit ELISA, MIP1-alpha Kit ELISA, Mip1a Kit ELISA, CCL3L1 Kit ELISA, CCL3L3 Kit ELISA, chemokine (C-C motif) ligand 3 Kit ELISA, C-C motif chemokine ligand 3 Kit ELISA, CCL3 Kit ELISA, Ccl3 Kit ELISA

ID gène: 6348

NCBI Accession: NP_002974

UniProt: A0N0R1

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy