N-Cadherin Kit ELISA

N° du produit ABIN6720014

Détail du produit N-Cadherin Kit ELISA

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CDw325, CDHN, Neural cadherin, N-cadherin, CD325

Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Gene Name: CDH2

Production: Natural and recombinant mouse Cadherin-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D160-A724

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Cadherin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Cadherin-2 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Autre désignation: Cadherin-2 - N-Cadherin (CDH2 Produits)

Synonymes: CD325 Kit ELISA, CDHN Kit ELISA, CDw325 Kit ELISA, NCAD Kit ELISA, N-cadherin Kit ELISA, cadherin-2 Kit ELISA, cdhn Kit ELISA, ncad Kit ELISA, cdw325 Kit ELISA, CDH2 Kit ELISA, Cadherin-2 Kit ELISA, Ncad Kit ELISA, glo Kit ELISA, lyr Kit ELISA, pac Kit ELISA, wu:fb47h04 Kit ELISA, cadherin 2 Kit ELISA, cadherin 2 S homeolog Kit ELISA, cadherin 2, type 1, N-cadherin (neuronal) Kit ELISA, CDH2 Kit ELISA, cdh2.S Kit ELISA, cdh2 Kit ELISA, Cdh2 Kit ELISA

ID gène: 12558

NCBI Accession: NP_031690

UniProt: P15116

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane