Thrombospondin 1 Kit ELISA

N° du produit ABIN6720022

Détail du produit Thrombospondin 1 Kit ELISA

Antigène: Thrombospondin 1 (THBS1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: THBS 1, THBS, THBS1, Thrombospondin 1, Thrombospondin-1, Tsp 1, TSP, Tsp1, TSP1_HUMAN

Background: Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

Gene Name: THBS1

Production: Natural and recombinant human THBS1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: N19-P1170

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human THBS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human THBS1 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 1.56 ng/mL-100 ng/mL

Other Performance Data: DOE: 2-8° C - 03/18/19 -20° C - 09/06/19

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Thrombospondin 1 (THBS1)

Autre désignation: THBS1 - TSP1 (THBS1 Produits)

Synonymes: THBS Kit ELISA, THBS-1 Kit ELISA, TSP Kit ELISA, TSP-1 Kit ELISA, TSP1 Kit ELISA, Thbs-1 Kit ELISA, tbsp1 Kit ELISA, thrombospondin-1 Kit ELISA, thbs Kit ELISA, thbs-1 Kit ELISA, tsp Kit ELISA, tsp-1 Kit ELISA, tsp1 Kit ELISA, Tsp1 Kit ELISA, THBS1 Kit ELISA, Tsp-1 Kit ELISA, wu:fc48c03 Kit ELISA, si:dkey-11e23.1 Kit ELISA, tsp2 Kit ELISA, thrombospondin 1 Kit ELISA, thrombospondin 1 S homeolog Kit ELISA, thrombospondin 1b Kit ELISA, thrombospondin 2 L homeolog Kit ELISA, THBS1 Kit ELISA, Thbs1 Kit ELISA, thbs1.S Kit ELISA, thbs1b Kit ELISA, thbs1 Kit ELISA, thbs2.L Kit ELISA

ID gène: 7057

NCBI Accession: NP_003237

UniProt: P07996

Pathways: Autophagy