CD97 Kit ELISA

N° du produit ABIN6720027

Détail du produit CD97 Kit ELISA

Antigène: CD97

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125 pg/mL - 8000 pg/mL

Seuil minimal de détection: 125 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD 97, CD_antigen=CD97, CD97, CD97 antigen, CD97 antigen subunit beta, CD97 Molecule, CD97_HUMAN, Leukocyte antigen CD97, Seven span transmembrane protein, Seven transmembrane helix receptor, Seven transmembrane heterodimeric receptor associated with inflammation, TM7LN 1, TM7LN1

Background: Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.

Gene Name: ADGRE5

Production: Natural and recombinant human CD97. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q21-Q398

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD97 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD97 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 125pg/mL-8000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CD97

Autre désignation: CD97 (CD97 Produits)

Synonymes: AA409984 Kit ELISA, TM7LN1 Kit ELISA, adhesion G protein-coupled receptor E5 Kit ELISA, Adgre5 Kit ELISA, ADGRE5 Kit ELISA

ID gène: 976

NCBI Accession: NP_001020331

UniProt: P48960