IL15RA Kit ELISA

N° du produit ABIN6720034

Détail du produit IL15RA Kit ELISA

Antigène: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125 pg/mL - 8000 pg/mL

Seuil minimal de détection: 125 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: AA690181, CD215, I15RA_HUMAN, IL 15R alpha, IL-15 receptor subunit alpha, IL-15R-alpha, IL-15RA, IL15RA, Interleukin 15 receptor alpha, Interleukin 15 receptor subunit alpha, MGC104179, sIL-15 receptor subunit alpha, sIL-15R-alpha, sIL-15RA, Soluble interleukin 15 receptor subunit alpha, Soluble interleukin-15 receptor subunit alpha

Background: Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.

Gene Name: IL15RA

Production: Natural and recombinant mouse IL15RA. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: G33-K205

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IL15RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL15RA standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 125pg/mL-8000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Autre désignation: IL15RA (IL15RA Produits)

Synonymes: IL15RA Kit ELISA, CD215 Kit ELISA, AA690181 Kit ELISA, IL-15RA Kit ELISA, interleukin 15 receptor subunit alpha Kit ELISA, interleukin 15 receptor, alpha chain Kit ELISA, IL15RA Kit ELISA, Il15ra Kit ELISA

ID gène: 16169

NCBI Accession: NP_001258426

UniProt: Q60819

Pathways: Signalistation JAK/STAT