CD40 Kit ELISA

N° du produit ABIN6720056

Détail du produit CD40 Kit ELISA

Antigène: CD40

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: B cell associated molecule|B cell associated molecule CD40, B cell surface antigen CD40, Bp50, CD40 antigen, CD40 Molecule, CD40 Molecule TNF receptor superfamily member 5, CD40 protein, CD40 type II isoform, CD40L receptor, Nerve growth factor receptor related B lymphocyte activation molecule, NFRSF5, p50, T BAM, TBAM, TNF receptor superfamily member 5, TNFRSF5, TRAP, Tumor necrosis factor receptor superfamily member 5, Tumor necrosis factor receptor superfamily member 5 precursor

Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Gene Name: CD40

Production: Natural and recombinant mouse CD40. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: V24-R193

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CD40 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD40 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CD40

Autre désignation: CD40 - TNFRSF5 (CD40 Produits)

Synonymes: Bp50 Kit ELISA, CDW40 Kit ELISA, TNFRSF5 Kit ELISA, p50 Kit ELISA, AI326936 Kit ELISA, GP39 Kit ELISA, HIGM1 Kit ELISA, IGM Kit ELISA, IMD3 Kit ELISA, T-BAM Kit ELISA, TRAP Kit ELISA, Tnfrsf5 Kit ELISA, TNFSF5 Kit ELISA, CD40 molecule Kit ELISA, CD40 antigen Kit ELISA, CD40 Kit ELISA, Cd40 Kit ELISA

ID gène: 21939

NCBI Accession: NP_035741

UniProt: P27512

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints