IL-27 Kit ELISA

N° du produit ABIN6720070

Détail du produit IL-27 Kit ELISA

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: IL 27A, IL 30, IL-27 subunit alpha, IL-27-A, IL27, IL27-A, IL27A, IL27A_HUMAN, IL27p28, IL30, Interleukin 27, Interleukin 27 subunit alpha, Interleukin 30, Interleukin-27 subunit alpha, p29

Background: Interleukin-27(IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus(EBV)-induced gene 3(EBI3)(also known as IL-27B) and IL27-p28(known as IL-30). IL-27 is produced by antigen-presenting cells. By genomic sequence analysis, the IL27 p28 gene was mapped to chromosome 16p11. L-27 plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130.

Gene Name: IL27

Production: Natural and recombinant mouse IL-27 p28. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: F29-S234

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-27 p28 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL-27 p28 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Autre désignation: IL-27 p28 (IL27 Produits)

Synonymes: IL-27 Kit ELISA, IL-27A Kit ELISA, IL27A Kit ELISA, IL27p28 Kit ELISA, IL30 Kit ELISA, p28 Kit ELISA, RGD1561420 Kit ELISA, IL27 Kit ELISA, IL-27p28 Kit ELISA, Il30 Kit ELISA, interleukin 27 Kit ELISA, IL27 Kit ELISA, Il27 Kit ELISA

ID gène: 246779

NCBI Accession: NP_663611

UniProt: Q8K3I6