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CD166 Kit ELISA

Rockland koa0683 ALCAM Reactivité: Souris Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
N° du produit ABIN6720077
N° du produit (Fournisseur): koa0683
  • Antigène Voir toutes CD166 (ALCAM) Kits ELISA
    CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
    Reactivité
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156 pg/mL - 10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    N° du produit (Fournisseur)
    koa0683
    Fournisseur
    Rockland
    Fonction
    Mouse ALCAM Sandwich ELISA Kit for Quantitative Detection
    Marque
    AccuSignal™
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificité

    Production: Natural and recombinant mouse ALCAM. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité
    < 10 pg/mL
    Ingrédients
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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  • Indications d'application
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse ALCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse ALCAM standard solution and each sample be measured in duplicate.
    Commentaires

    Standard: Expression system for standard: NSO, Immunogen sequence: W28-K527

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Date de péremption
    12 months
  • Antigène Voir toutes CD166 (ALCAM) Kits ELISA
    CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
    Autre désignation
    ALCAM (ALCAM Produits)
    Sujet

    Synonyms: Activated leukocyte cell adhesion molecule, ALCAM, ALCAM protein, CD 166, CD166, CD166 antigen, CD166_HUMAN, FLJ3851, FLJ38514, MEMD, MGC71733

    Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

    ID gène
    11658
    NCBI Accession
    NP_033785
    UniProt
    Q61490
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