CD166 Kit ELISA
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- Antigène Voir toutes CD166 (ALCAM) Kits ELISA
- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 156 pg/mL - 10000 pg/mL
- Seuil minimal de détection
- 156 pg/mL
- Application
- ELISA
- N° du produit (Fournisseur)
- koa0683
- Fournisseur
- Rockland
- Fonction
- Mouse ALCAM Sandwich ELISA Kit for Quantitative Detection
- Marque
- AccuSignal™
- Type d'échantillon
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificité
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Production: Natural and recombinant mouse ALCAM. There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- < 10 pg/mL
- Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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- Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse ALCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse ALCAM standard solution and each sample be measured in duplicate.
- Commentaires
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Standard: Expression system for standard: NSO, Immunogen sequence: W28-K527
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Date de péremption
- 12 months
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- Antigène Voir toutes CD166 (ALCAM) Kits ELISA
- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
- Autre désignation
- ALCAM (ALCAM Produits)
- Sujet
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Synonyms: Activated leukocyte cell adhesion molecule, ALCAM, ALCAM protein, CD 166, CD166, CD166 antigen, CD166_HUMAN, FLJ3851, FLJ38514, MEMD, MGC71733
Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
- ID gène
- 11658
- NCBI Accession
- NP_033785
- UniProt
- Q61490
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