Cardiotrophin 1 Kit ELISA

N° du produit ABIN6720095

Détail du produit Cardiotrophin 1 Kit ELISA

Antigène: Cardiotrophin 1 (CTF1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Cardiophin 1, Cardiophin1, Cardiotrophin I, Cardiotrophin-1, CardiotrophinI, CT 1, CT-1, CT1, CTF 1, Ctf1, CTF1_HUMAN

Background: Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80 % amino acid identity with the 203-amino acid mouse CT-1 sequence, however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidn...

Gene Name: CTF1

Production: Natural and recombinant human CT-1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: S2-A201

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CT-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CT-1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Cardiotrophin 1 (CTF1)

Autre désignation: Cardiotrophin-1 (CTF1 Produits)

Synonymes: CTF1 Kit ELISA, ct1 Kit ELISA, ct-1 Kit ELISA, ctf2 Kit ELISA, CT-1 Kit ELISA, CT1 Kit ELISA, cardiotrophin 1 Kit ELISA, cardiotrophin-1 Kit ELISA, CTF1 Kit ELISA, ctf1 Kit ELISA, Ctf1 Kit ELISA, LOC100736818 Kit ELISA

ID gène: 1489

NCBI Accession: NP_001136016

UniProt: Q16619

Pathways: Signalistation JAK/STAT