Cardiotrophin 1 Kit ELISA

N° du produit ABIN6720095

N° du produit (Fournisseur): koa0342

Détail du produit Cardiotrophin 1 Kit ELISA

Antigène: Cardiotrophin 1 (CTF1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

N° du produit (Fournisseur): koa0342

Fournisseur: Rockland

Fonction: Human Cardiotrophin-1 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human CT-1. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CT-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CT-1 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: S2-A201

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Cardiotrophin 1 (CTF1)

Autre désignation: Cardiotrophin-1 (CTF1 Produits)

Sujet:

Synonyms: Cardiophin 1, Cardiophin1, Cardiotrophin I, Cardiotrophin-1, CardiotrophinI, CT 1, CT-1, CT1, CTF 1, Ctf1, CTF1_HUMAN

Background: Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80 % amino acid identity with the 203-amino acid mouse CT-1 sequence, however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidn...

ID gène: 1489

NCBI Accession: NP_001136016

UniProt: Q16619

Pathways: Signalistation JAK/STAT