Galectin 3 Kit ELISA

N° du produit ABIN6720103

Détail du produit Galectin 3 Kit ELISA

Antigène: Galectin 3 (LGALS3)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, Galectin-3, GALIG, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, lectin, galactoside-binding, soluble, 3, LGALS2Mac-2 antigen, MAC2, Macrophage galactose-specific lectin

Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Gene Name: LGALS3

Production: Natural and recombinant human Galectin-3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A2-I250

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Galectin-3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Galectin 3 (LGALS3)

Autre désignation: Galectin-3 - LGALS3 (LGALS3 Produits)

Synonymes: CBP 35 Kit ELISA, Gal-3 Kit ELISA, Galectin-3 Kit ELISA, leg3 Kit ELISA, galactoside-binding Kit ELISA, galectin-3 Kit ELISA, lectin Kit ELISA, soluble Kit ELISA, CBP35 Kit ELISA, GAL3 Kit ELISA, GALBP Kit ELISA, GALIG Kit ELISA, L31 Kit ELISA, LGALS2 Kit ELISA, MAC2 Kit ELISA, MGC52947 Kit ELISA, MGC75803 Kit ELISA, LGALS3 Kit ELISA, gal-3 Kit ELISA, GBP Kit ELISA, L-34 Kit ELISA, Mac-2 Kit ELISA, gal3 Kit ELISA, galectin 3 Kit ELISA, Galectin-3 Kit ELISA, avian beta-defensin 3 Kit ELISA, lectin, galactoside-binding, soluble, 3 L homeolog Kit ELISA, lectin, galactoside-binding, soluble, 3 Kit ELISA, galectin 2 Kit ELISA, lectin, galactose binding, soluble 3 Kit ELISA, LGALS3 Kit ELISA, leg3 Kit ELISA, AvBD3 Kit ELISA, lgals3.L Kit ELISA, lgals3 Kit ELISA, LGALS2 Kit ELISA, Lgals3 Kit ELISA

Classe de substances: Chemical

ID gène: 3958

NCBI Accession: NP_002297

UniProt: P17931

Pathways: Signalisation RTK