KIT Kit ELISA
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- Antigène Voir toutes KIT Kits ELISA
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 156 pg/mL - 10000 pg/mL
- Seuil minimal de détection
- 156 pg/mL
- Application
- ELISA
- N° du produit (Fournisseur)
- koa0501
- Fournisseur
- Rockland
- Fonction
- Human KIT - SCFR Sandwich ELISA Kit for Quantitative Detection
- Marque
- AccuSignal™
- Type d'échantillon
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificité
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Production: Natural and recombinant human KIT. There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- < 10 pg/mL
- Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product KIT Kit ELISA
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- Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human KIT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KIT standard solution and each sample be measured in duplicate.
- Commentaires
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Standard: Expression system for standard: sf21, Immunogen sequence: Q26-T520
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Date de péremption
- 12 months
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- Antigène Voir toutes KIT Kits ELISA
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- Autre désignation
- KIT/SCFR (KIT Produits)
- Sujet
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Synonyms: CD117, CD117 antigen, EC 2.7.10.1, Mast/stem cell growth factor receptor precursor, PBT, Proto oncogene tyrosine protein kinase Kit, SCFR, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog
Background: SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
- ID gène
- 3815
- NCBI Accession
- NP_000213
- UniProt
- P10721
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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