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KIT Kit ELISA

Rockland koa0501 KIT Reactivité: Humain Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
N° du produit ABIN6720112
N° du produit (Fournisseur): koa0501
  • Antigène Voir toutes KIT Kits ELISA
    KIT (Mast/stem Cell Growth Factor Receptor (KIT))
    Reactivité
    • 15
    • 10
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156 pg/mL - 10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    N° du produit (Fournisseur)
    koa0501
    Fournisseur
    Rockland
    Fonction
    Human KIT - SCFR Sandwich ELISA Kit for Quantitative Detection
    Marque
    AccuSignal™
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificité

    Production: Natural and recombinant human KIT. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité
    < 10 pg/mL
    Ingrédients
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product KIT Kit ELISA
  • Indications d'application
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human KIT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KIT standard solution and each sample be measured in duplicate.
    Commentaires

    Standard: Expression system for standard: sf21, Immunogen sequence: Q26-T520

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Date de péremption
    12 months
  • Antigène Voir toutes KIT Kits ELISA
    KIT (Mast/stem Cell Growth Factor Receptor (KIT))
    Autre désignation
    KIT/SCFR (KIT Produits)
    Sujet

    Synonyms: CD117, CD117 antigen, EC 2.7.10.1, Mast/stem cell growth factor receptor precursor, PBT, Proto oncogene tyrosine protein kinase Kit, SCFR, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog

    Background: SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

    ID gène
    3815
    NCBI Accession
    NP_000213
    UniProt
    P10721
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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