TEK Kit ELISA

N° du produit ABIN6720115

Détail du produit TEK Kit ELISA

Antigène: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Angiopoietin-1 receptor, CD202b, CD202b antigen, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, Tek, TEK tyrosine kinase, endothelial, TIE 2, TIE2_HUMAN, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1

Background: Tyrosine kinase with Ig and EGF homology domain 2(Tie-2), also called TEK tyrosine kinase, endothelial(TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesi...

Gene Name: TEK

Production: Natural and recombinant human TIE2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A23-K745

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIE2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TIE2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Autre désignation: TIE2 (TEK Produits)

Synonymes: TEK Kit ELISA, DKFZp469E064 Kit ELISA, AA517024 Kit ELISA, Cd202b Kit ELISA, Hyk Kit ELISA, Tie2 Kit ELISA, tie-2 Kit ELISA, CD202B Kit ELISA, TIE-2 Kit ELISA, TIE2 Kit ELISA, VMCM Kit ELISA, VMCM1 Kit ELISA, Tie-2 Kit ELISA, tie2 Kit ELISA, TEK receptor tyrosine kinase Kit ELISA, angiopoietin-1 receptor Kit ELISA, TEK tyrosine kinase, endothelial Kit ELISA, TEK Kit ELISA, LOC100408334 Kit ELISA, tek Kit ELISA, Tek Kit ELISA

ID gène: 7010

NCBI Accession: NP_000450

UniProt: Q02763

Pathways: Signalisation RTK, Growth Factor Binding