Leptin Receptor Kit ELISA

N° du produit ABIN6720133

Détail du produit Leptin Receptor Kit ELISA

Antigène: Leptin Receptor (LEPR)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD295, HuB220, LEP-R, LEPR, LEPR_HUMAN, Leptin receptor, OB receptor, OB-R, OBR

Background: Leptin receptor(or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene(LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.

Gene Name: LEPR

Production: Natural and recombinant mouse Leptin receptor. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence:  L22-G839

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Leptin receptor standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Leptin receptor standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Leptin Receptor (LEPR)

Autre désignation: Leptin receptor (LEPR Produits)

Synonymes: CD295 Kit ELISA, LEP-R Kit ELISA, LEPRD Kit ELISA, OB-R Kit ELISA, OBR Kit ELISA, obr Kit ELISA, cd295 Kit ELISA, ob-rb Kit ELISA, Fa Kit ELISA, LEPROT Kit ELISA, Leprb Kit ELISA, Modb1 Kit ELISA, OB-RGRP Kit ELISA, Obr Kit ELISA, db Kit ELISA, diabetes Kit ELISA, obese-like Kit ELISA, obl Kit ELISA, ObRb Kit ELISA, Ob-R Kit ELISA, Ob-RM Kit ELISA, lepr Kit ELISA, leptin receptor Kit ELISA, leptin receptor S homeolog Kit ELISA, LEPR Kit ELISA, lepr Kit ELISA, Lepr Kit ELISA, lepr.S Kit ELISA

ID gène: 16847

NCBI Accession: NP_001116371

UniProt: P48356

Pathways: Signalistation JAK/STAT, AMPK Signaling, Feeding Behaviour