Myeloperoxidase Kit ELISA

N° du produit ABIN6720134

Détail du produit Myeloperoxidase Kit ELISA

Antigène: Myeloperoxidase (MPO)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: 84 kDa myeloperoxidase, 89 kDa myeloperoxidase, MPO, MPO Myeloperoxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain, PERM_HUMAN

Background: Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Gene Name: MPO

Production: Natural and recombinant mouse MPO. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: M16-T718

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse MPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse MPO standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Other Performance Data: DOE: 2-8° C- 2/7/18 -20° C - 8/8/18

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Myeloperoxidase (MPO)

Autre désignation: MPO (MPO Produits)

Synonymes: mKIAA4033 Kit ELISA, MPO Kit ELISA, LOC100335032 Kit ELISA, POX2' Kit ELISA, XPOX2' Kit ELISA, mpo Kit ELISA, mpo-A Kit ELISA, pmr-1 Kit ELISA, pmr1 Kit ELISA, pox2 Kit ELISA, xpox2 Kit ELISA, myeloperoxidase Kit ELISA, myeloperoxidase L homeolog Kit ELISA, MPO Kit ELISA, Mpo Kit ELISA, LOC100335032 Kit ELISA, mpo.L Kit ELISA

ID gène: 17523

NCBI Accession: NP_034954

UniProt: P11247

Pathways: Chromatin Binding