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CCL22 Kit ELISA

Rockland koa0255 CCL22 Reactivité: Humain Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
N° du produit ABIN6720151
N° du produit (Fournisseur): koa0255
  • Antigène Voir toutes CCL22 Kits ELISA
    CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))
    Reactivité
    • 14
    • 11
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    N° du produit (Fournisseur)
    koa0255
    Fournisseur
    Rockland
    Fonction
    Human CCL22 - MDC Sandwich ELISA Kit for Quantitative Detection
    Marque
    AccuSignal™
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificité

    Production: Natural and recombinant human MDC. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité
    < 1 pg/mL
    Ingrédients
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product CCL22 Kit ELISA
  • Indications d'application
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human MDC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MDC standard solution and each sample be measured in duplicate.
    Commentaires

    Standard: Expression system for standard: E.coli, Immunogen sequence:  G25-Q93

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Date de péremption
    12 months
  • Antigène Voir toutes CCL22 Kits ELISA
    CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))
    Autre désignation
    CCL22/MDC (CCL22 Produits)
    Sujet

    Synonyms: A-152E5.1, ABCD 1, ABCD1, C-C motif chemokine 22, CC chemokine STCP-1, Ccl22, CCL22_HUMAN, Chemokine (C-C motif) ligand 22, DC/B-CK, Macrophage-derived chemokine, MDC(1-69), MDC(7-69), MGC34554, SCYA22, Small inducible cytokine A22, Small inducible cytokine subfamily A (Cys-Cys), member 22, Small-inducible cytokine A22, STCP 1, STCP1, Stimulated T cell chemotactic protein 1, Stimulated T-cell chemotactic protein 1

    Background: Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37 % identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35 % identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

    ID gène
    6367
    NCBI Accession
    NP_002981
    UniProt
    O00626
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