CCL22 Kit ELISA

N° du produit ABIN6720151

N° du produit (Fournisseur): koa0255

Détail du produit CCL22 Kit ELISA

Antigène: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

N° du produit (Fournisseur): koa0255

Fournisseur: Rockland

Fonction: Human CCL22 - MDC Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human MDC. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human MDC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MDC standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence:  G25-Q93

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Autre désignation: CCL22/MDC (CCL22 Produits)

Sujet:

Synonyms: A-152E5.1, ABCD 1, ABCD1, C-C motif chemokine 22, CC chemokine STCP-1, Ccl22, CCL22_HUMAN, Chemokine (C-C motif) ligand 22, DC/B-CK, Macrophage-derived chemokine, MDC(1-69), MDC(7-69), MGC34554, SCYA22, Small inducible cytokine A22, Small inducible cytokine subfamily A (Cys-Cys), member 22, Small-inducible cytokine A22, STCP 1, STCP1, Stimulated T cell chemotactic protein 1, Stimulated T-cell chemotactic protein 1

Background: Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37 % identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35 % identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

ID gène: 6367

NCBI Accession: NP_002981

UniProt: O00626