CD73 Kit ELISA

N° du produit ABIN6720156

Détail du produit CD73 Kit ELISA

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: 5' NT, 5' nucleotidase (CD73)|5' nucleotidase precursor, 5' nucleotidase ecto, 5' nucleotidase ecto (CD73), 5'-NT, 5'-nucleotidase, 5NTD_HUMAN, CD73, CD73 antigen, E5NT, Ecto 5' nucleotidase, Ecto-5'-nucleotidase, eN, eNT, NT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase

Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Gene Name: NT5E

Production: Natural and recombinant human CD73. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: W27-K547

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CD73 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CD73 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Autre désignation: CD73 - NT5E (NT5E Produits)

Synonymes: CALJA Kit ELISA, CD73 Kit ELISA, E5NT Kit ELISA, NT Kit ELISA, NT5 Kit ELISA, NTE Kit ELISA, eN Kit ELISA, eNT Kit ELISA, zgc:63784 Kit ELISA, NT5E Kit ELISA, MGC114869 Kit ELISA, PSPTO2277 Kit ELISA, 2210401F01Rik Kit ELISA, AI447961 Kit ELISA, Nt5 Kit ELISA, 5'-nucleotidase ecto Kit ELISA, 5'-nucleotidase, ecto (CD73) Kit ELISA, 5'-nucleotidase Kit ELISA, 5' nucleotidase, ecto Kit ELISA, NT5E Kit ELISA, nt5e Kit ELISA, nt5e.S Kit ELISA, PSPTO_2277 Kit ELISA, CBU_1233 Kit ELISA, MCA1322 Kit ELISA, Nt5e Kit ELISA

ID gène: 4907

NCBI Accession: NP_001191742

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process