DIABLO Kit ELISA

N° du produit ABIN6720163

Détail du produit DIABLO Kit ELISA

Antigène: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: DFNA64, DIABLO, diablo homolog, mitochondrial, diablo, IAP-binding mitochondrial protein, DIABLO-S, direct IAP-binding protein with low pI, mitochondrial Smac protein, second mitochondria-derived activator of caspase, SMAC3

Background: Diablo homolog, mitochondrial, also known as second mitochondria-derived activator of caspases(SMAC), is a protein that in humans is encoded by the DIABLO gene(direct IAP binding protein with low pI). It is mapped to 12q24.31. DIABLO can bind mammalian IAP homolog A(MIHA, or API3) and can also interact with MIHB, MIHC, and OpIAP, the baculoviral IAP. This gene encodes an inhibitor of apoptosis protein(IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Over expression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64.

Gene Name: DIABLO

Production: Natural and recombinant human Diablo. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A56-D239

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Diablo standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Diablo standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Autre désignation: Diablo - SMAC (DIABLO Produits)

Synonymes: smac Kit ELISA, smac3 Kit ELISA, diablo-s Kit ELISA, 0610041G12Rik Kit ELISA, 1700006L01Rik Kit ELISA, AU040403 Kit ELISA, Smac Kit ELISA, DFNA64 Kit ELISA, SMAC Kit ELISA, DBO Kit ELISA, XSmac Kit ELISA, diablo, IAP-binding mitochondrial protein Kit ELISA, diablo IAP-binding mitochondrial protein Kit ELISA, diablo, IAP-binding mitochondrial protein L homeolog Kit ELISA, diablo Kit ELISA, Diablo Kit ELISA, DIABLO Kit ELISA, diablo.L Kit ELISA

ID gène: 56616

NCBI Accession: NP_001265231

UniProt: Q9NR28

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity