PI3 Kit ELISA
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- Antigène Voir toutes PI3 Kits ELISA
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 31.2 pg/mL - 2000 pg/mL
- Seuil minimal de détection
- 31.2 pg/mL
- Application
- ELISA
- N° du produit (Fournisseur)
- koa0637
- Fournisseur
- Rockland
- Fonction
- Human Elafin - PI3 Sandwich ELISA Kit for Quantitative Detection
- Marque
- AccuSignal™
- Type d'échantillon
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificité
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Production: Natural and recombinant human Elafin. There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- < 10 pg/mL
- Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product PI3 Kit ELISA
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- Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Elafin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human Elafin standard solution and each sample be measured in duplicate.
- Commentaires
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Standard: Expression system for standard: NSO, Immunogen sequence: A23-Q117
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Date de péremption
- 12 months
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- Antigène Voir toutes PI3 Kits ELISA
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
- Autre désignation
- Elafin/PI3 (PI3 Produits)
- Sujet
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Synonyms: Cementoin, Elafin, Elastase specific inhibitor, ESI, MGC13613, Peptidase inhibitor 3, Peptidase inhibitor 3 skin derived, PI 3, PI3, Pre elafin, Protease inhibitor WAP3, SKALP, Skin derived antileukoproteinase, Trappin 2, WAP four disulfide core domain 14, WAP four disulfide core domain protein 14, WAP3, WFDC14
Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase(SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core(WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
- ID gène
- 5266
- NCBI Accession
- NP_002629
- UniProt
- P19957
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