NOV Kit ELISA

N° du produit ABIN6720176

Détail du produit NOV Kit ELISA

Antigène: NOV (Nephroblastoma Overexpressed (NOV))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CCN family member 3, CCN3, IBP-9, IGF-binding protein 9, IGFBP-9, IGFBP9, Insulin-like growth factor-binding protein 9, Nephroblastoma overexpressed, Nephroblastoma overexpressed gene protein homolog, Nephroblastoma-overexpressed gene protein homolog, NOV, NOV_HUMAN, NovH, Protein NOV homolog

Background: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.

Gene Name: NOV

Production: Natural and recombinant human NOV. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: T32-M357

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NOV standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human NOV standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Other Performance Data: DOE: 2-8 C - 11/23/17 -20 C - 05/24/18

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: NOV (Nephroblastoma Overexpressed (NOV))

Autre désignation: NOV - CCN3 (NOV Produits)

Synonymes: CCN3 Kit ELISA, IBP-9 Kit ELISA, IGFBP-9 Kit ELISA, IGFBP9 Kit ELISA, NOVh Kit ELISA, nov-A Kit ELISA, xnov Kit ELISA, NovH Kit ELISA, C130088N23Rik Kit ELISA, nephroblastoma overexpressed Kit ELISA, nephroblastoma overexpressed L homeolog Kit ELISA, nephroblastoma overexpressed gene Kit ELISA, NOV Kit ELISA, nov.L Kit ELISA, Nov Kit ELISA

ID gène: 4856

NCBI Accession: NP_002505

UniProt: P48745

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding