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Stanniocalcin 1 Kit ELISA

STC1 Reactivité: Humain Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
N° du produit ABIN6720178
  • Antigène Voir toutes Stanniocalcin 1 (STC1) Kits ELISA
    Stanniocalcin 1 (STC1)
    Reactivité
    • 4
    • 2
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: Stanniocalcin, Stanniocalcin-1, STC, STC-1, STC1, STC1_HUMAN

    Background: Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.

    Gene Name: STC1

    Production: Natural and recombinant human STC1. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: V34-A247

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    Discover our top product STC1 Kit ELISA
  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human STC1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human STC1 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2pg/mL-2000pg/mL

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène Voir toutes Stanniocalcin 1 (STC1) Kits ELISA
    Stanniocalcin 1 (STC1)
    Autre désignation
    Stanniocalcin 1 - STC1 (STC1 Produits)
    Synonymes
    STC Kit ELISA, Stc Kit ELISA, stc1 Kit ELISA, MGC82280 Kit ELISA, STC1 Kit ELISA, zgc:153307 Kit ELISA, LOC777959 Kit ELISA, stanniocalcin 1 Kit ELISA, stanniocalcin 1 L homeolog Kit ELISA, stanniocalcin 1 S homeolog Kit ELISA, STC1 Kit ELISA, Stc1 Kit ELISA, stc1.L Kit ELISA, stc1.S Kit ELISA, stc1 Kit ELISA, LOC777959 Kit ELISA
    ID gène
    6781
    NCBI Accession
    NP_003146
    UniProt
    P52823
    Pathways
    Hormone Activity
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