CCL28 Kit ELISA

N° du produit ABIN6720187

Détail du produit CCL28 Kit ELISA

Antigène: CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CCK 1, CCK1, CCL 28, Chemokine (C-C motif) ligand 28, MEC, MGC71902, Mucosae associated epithelial chemokine, OTTHUMP0000022162, SCYA28, Small inducible cytokine A28, Small inducible cytokine A28 [Precursor], Small inducible cytokine subfamily A (Cys-Cys), member 28

Background: CCL28, also known as mucosae-associated epithelial chemokine(MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T(Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

Gene Name: CCL28

Production: Natural and recombinant mouse CCL28. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: I23-R130

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CCL28 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL28 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))

Autre désignation: CCL28 (CCL28 Produits)

Synonymes: Scya28 Kit ELISA, CCL28 Kit ELISA, ccl28 Kit ELISA, nrpb Kit ELISA, enc-1 Kit ELISA, pig10 Kit ELISA, Encr-3 Kit ELISA, klhl35 Kit ELISA, Xencr-3 Kit ELISA, tp53i10 Kit ELISA, LOC100303767 Kit ELISA, CCK1 Kit ELISA, MEC Kit ELISA, SCYA28 Kit ELISA, C-C motif chemokine ligand 28 Kit ELISA, ectodermal-neural cortex 1 (with BTB domain) Kit ELISA, C-C motif chemokine 28 Kit ELISA, chemokine (C-C motif) ligand 28 Kit ELISA, Ccl28 Kit ELISA, CCL28 Kit ELISA, ccl28 Kit ELISA, enc1 Kit ELISA, LOC100303767 Kit ELISA

ID gène: 56838

NCBI Accession: NP_064675

UniProt: Q9JIL2