Azurocidin Kit ELISA

N° du produit ABIN6720212

Détail du produit Azurocidin Kit ELISA

Antigène: Azurocidin (AZU1) (Azurocidin 1 (AZU1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Azu 1, AZU1, Azurocidin, CAP37, CAP7_HUMAN, Cationic antimicrobial protein 37, Cationic antimicrobial protein CAP37, HBP, Heparin binding protein, Heparin-binding protein, HUMAZUR, Neutrophil azurocidin

Background: Azurocidin, also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP), is a protein that in humans is encoded by the AZU1 gene. This encoded protein is a member of the serine protease gene family, but it is not a serine proteinase, because the active site serine and histidine residues are replaced. Azurocidin is mapped to 19p13.3. The protein encoded by this gene is an azurophil granule antibiotic protein, with antibacterial activity. It is also an important multifunctional inflammatory mediator. In addition to it, Azurocidin is also a specific chemoattractant for monocytes. It lacks the chemotactic activity for neutrophils and lymphocytes, and this gene is probably responsible for the wave of monocytes that follows the initial wave of PMNs typical of the inflammatory response.

Gene Name: AZU1

Production: Natural and recombinant human Azurocidin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: I27-P248

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Azurocidin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Azurocidin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Azurocidin (AZU1) (Azurocidin 1 (AZU1))

Autre désignation: Azurocidin (AZU1 Produits)

Synonymes: AZAMP Kit ELISA, AZU Kit ELISA, CAP37 Kit ELISA, HBP Kit ELISA, HUMAZUR Kit ELISA, NAZC Kit ELISA, AZU1 Kit ELISA, Azurocidin Kit ELISA, pHBP Kit ELISA, azurocidin 1 Kit ELISA, azurocidin Kit ELISA, AZU1 Kit ELISA, CpipJ_CPIJ007384 Kit ELISA

ID gène: 566

NCBI Accession: NP_001691

UniProt: P20160