c-MET Kit ELISA

N° du produit ABIN6720214

Détail du produit c-MET Kit ELISA

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: AUTS9, c Met, Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, HGFR, MET, Met proto oncogene, Met proto oncogene tyrosine kinase, MET_HUMAN, Oncogene MET, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met

Background: C-Met(MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor(HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.

Gene Name: MET

Production: Natural and recombinant human C-MET. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E25-R307(alpha)&S308-T932(beta)

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human C-MET standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human C-MET standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Autre désignation: C-MET - HGFR (MET Produits)

Synonymes: AUTS9 Kit ELISA, HGFR Kit ELISA, RCCP2 Kit ELISA, c-Met Kit ELISA, AI838057 Kit ELISA, HGF Kit ELISA, Par4 Kit ELISA, Hgfr Kit ELISA, c-met Kit ELISA, MET Kit ELISA, C-MET Kit ELISA, met Kit ELISA, met-A Kit ELISA, MET proto-oncogene, receptor tyrosine kinase Kit ELISA, met proto-oncogene Kit ELISA, MET proto-oncogene, receptor tyrosine kinase L homeolog Kit ELISA, MET Kit ELISA, Met Kit ELISA, met.L Kit ELISA

ID gène: 4233

NCBI Accession: NP_000236

UniProt: P08581

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor