Cathepsin L Kit ELISA

N° du produit ABIN6720215

Détail du produit Cathepsin L Kit ELISA

Antigène: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Cathepsin L1, Cathepsin L1 light chain, CathepsinL, CATL, CATL1_HUMAN, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP

Background: Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.

Gene Name: CTSL

Production: Natural and recombinant human Cathepsin L. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E113-V333

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Cathepsin L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Cathepsin L standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Other Performance Data: DOE: 2-8° C - 04/25/18 -20° C - 10/26/18

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Autre désignation: Cathepsin L (CTSL1 Produits)

Synonymes: CATL Kit ELISA, CTSL1 Kit ELISA, MEP Kit ELISA, 1190035F06Rik Kit ELISA, Ctsl1 Kit ELISA, fs Kit ELISA, nkt Kit ELISA, CATHL Kit ELISA, CatL Kit ELISA, Ctsl Kit ELISA, CTSL Kit ELISA, mep Kit ELISA, catl Kit ELISA, ctsl Kit ELISA, cpl-1 Kit ELISA, MGC80629 Kit ELISA, MGC107932 Kit ELISA, LOC663234 Kit ELISA, 26-29-p Kit ELISA, Cp1 Kit ELISA, si:dkey-121a11.6 Kit ELISA, CTSV Kit ELISA, catL Kit ELISA, cb15 Kit ELISA, ctsl1b Kit ELISA, hgg1 Kit ELISA, wu:fb30g09 Kit ELISA, zgc:92089 Kit ELISA, cathepsin L Kit ELISA, cathepsin V Kit ELISA, cathepsin L L homeolog Kit ELISA, cathepsin L1 Kit ELISA, Cathepsin L1 Kit ELISA, Cathepsin L Kit ELISA, testin gene Kit ELISA, cathepsin 12 Kit ELISA, cathepsin Lb Kit ELISA, cathepsin L.1 Kit ELISA, CTSL Kit ELISA, Ctsl Kit ELISA, CTSV Kit ELISA, ctsl.L Kit ELISA, ctsl Kit ELISA, LOC100008830 Kit ELISA, catl1 Kit ELISA, 26-29-p Kit ELISA, catl Kit ELISA, Tsp_11596 Kit ELISA, Testin Kit ELISA, cts12 Kit ELISA, LOC102156614 Kit ELISA, ctslb Kit ELISA, ctsl.1 Kit ELISA

ID gène: 1514

NCBI Accession: NP_001244900

UniProt: P07711

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades