2B4 Kit ELISA

N° du produit ABIN6720218

N° du produit (Fournisseur): koa0562

Détail du produit 2B4 Kit ELISA

Antigène: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

N° du produit (Fournisseur): koa0562

Fournisseur: Rockland

Fonction: Human CD244 - 2B4 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human CD244. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CD244 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD244 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: C22-R221

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Autre désignation: CD244/2B4 (CD244 Produits)

Sujet:

Synonyms: 2B4, C9.1, CD244, CD244 antigen, CD244 Molecule natural killer cell receptor 2B4, CD244 natural killer cell receptor 2B4, CD244_HUMAN, h2B4, Ly90, Natural killer cell activation-inducing ligand, Natural killer cell receptor 2B4, NK cell activation inducing ligand, NK cell activation inducing ligand NAIL, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, NKR2B4, Nmrk, Non-MHC restricted killing associated, p38, signaling lymphocytic activation molecule 4, SLAM family member 4, SLAMF4

Background: CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead, the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.

ID gène: 51744

NCBI Accession: NP_001160135

UniProt: Q9BZW8