CSTB Kit ELISA

N° du produit ABIN6720224

Détail du produit CSTB Kit ELISA

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CHROW21, CPI B, CPI-B, CST6, CSTB, Cystatin B, Cystatin-B, CYTB, CYTB_HUMAN, EPM1, EPM1A, Liver thiol proteinase inhibitor, PME, Stefin-B, STFB, ULD

Background: Cystatin B(CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

Gene Name: CSTB

Production: Natural and recombinant human Cystatin B. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: M2-F98

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Cystatin B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. We recommend that each human Cystatin B standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Autre désignation: Cystatin B (CSTB Produits)

Synonymes: CST6 Kit ELISA, EPM1 Kit ELISA, EPM1A Kit ELISA, PME Kit ELISA, STFB Kit ELISA, ULD Kit ELISA, CSTB Kit ELISA, Cyb Kit ELISA, Epm1 Kit ELISA, Stfb Kit ELISA, MGC189005 Kit ELISA, pme Kit ELISA, cst6 Kit ELISA, epm1 Kit ELISA, stfb Kit ELISA, AA960480 Kit ELISA, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 Kit ELISA, ATCYS6 Kit ELISA, ATCYSB Kit ELISA, cystatin B Kit ELISA, LOC100008600 Kit ELISA, LOC100136235 Kit ELISA, CPI1 Kit ELISA, cystatin B Kit ELISA, cystatin B (stefin B) Kit ELISA, cystatin 14a, tandem duplicate 2 Kit ELISA, cystatin-B Kit ELISA, CSTB Kit ELISA, Cstb Kit ELISA, cstb Kit ELISA, cst14a.2 Kit ELISA, CYSB Kit ELISA, cpi1 Kit ELISA, LOC101123265 Kit ELISA

ID gène: 1476

NCBI Accession: NP_000091

UniProt: P04080

Pathways: Response to Water Deprivation