DKK1 Kit ELISA

N° du produit ABIN6720225

Détail du produit DKK1 Kit ELISA

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Dickkopf 1, Dickkopf homolog 1, Dickkopf related protein 1, DKK 1, hDkk 1, hDkk1, mDkk1, SK

Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Gene Name: DKK1

Production: Oligomeric forms of natural and recombinant human DKK1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: T32-H266

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human DKK1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Autre désignation: DKK-1 (DKK1 Produits)

Synonymes: DKK-1 Kit ELISA, SK Kit ELISA, AI414495 Kit ELISA, SGY Kit ELISA, SGY-1 Kit ELISA, SGY1 Kit ELISA, Soggy Kit ELISA, mSgy Kit ELISA, dkk1 Kit ELISA, dkk Kit ELISA, dkk-1 Kit ELISA, xdkk1 Kit ELISA, Xdkk-1 Kit ELISA, Dickkopf1 Kit ELISA, dickkopf-1 Kit ELISA, mdkk-1 Kit ELISA, dkk1-A Kit ELISA, dickkopf WNT signaling pathway inhibitor 1 Kit ELISA, dickkopf like acrosomal protein 1 Kit ELISA, dickkopf-related protein 1 Kit ELISA, dickkopf-like 1 Kit ELISA, dickkopf WNT signaling pathway inhibitor 1b Kit ELISA, dickkopf protein Kit ELISA, dickkopf WNT signaling pathway inhibitor 1 S homeolog Kit ELISA, DKK1 Kit ELISA, Dkk1 Kit ELISA, DKKL1 Kit ELISA, LOC100228789 Kit ELISA, Dkkl1 Kit ELISA, dkk1b Kit ELISA, dkk1 Kit ELISA, dickkopf Kit ELISA, dkk1.S Kit ELISA

ID gène: 83430

NCBI Accession: NP_112542

UniProt: Q9EQ14

Pathways: Signalisation WNT, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation