LTA Kit ELISA
-
- Antigène Voir toutes LTA Kits ELISA
- LTA (Lymphotoxin-alpha (LTA))
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 62.5 pg/mL - 4000 pg/mL
- Seuil minimal de détection
- 62.5 pg/mL
- Application
- ELISA
- N° du produit (Fournisseur)
- koa0358
- Fournisseur
- Rockland
- Fonction
- Human TNF beta Sandwich ELISA Kit for Quantitative Detection
- Marque
- AccuSignal™
- Type d'échantillon
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificité
-
Production: Natural and recombinant human TNF beta. There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- < 5 pg/mL
- Ingrédients
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product LTA Kit ELISA
-
-
- Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNF beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human TNF beta standard solution and each sample be measured in duplicate.
- Commentaires
-
Standard: Expression system for standard: E.coli, Immunogen sequence: L35-L205
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
-
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Date de péremption
- 12 months
-
- Antigène Voir toutes LTA Kits ELISA
- LTA (Lymphotoxin-alpha (LTA))
- Autre désignation
- TNF beta (LTA Produits)
- Classe de substances
- Chemical
- Sujet
-
Synonyms: Coley's toxin, DAMA-25N12.13-004, DIF, Differentiation inducing factor, Hemorrhagic factor, LT, LT alpha, LT-alpha, Lta, LTalpha, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Lymphotoxin-alpha, Natural killer cytotoxic factor, Necrosin, NKCF, TNF B, TNF superfamily member 1, TNF-beta, TNFB, TNFB_HUMAN, TNFbeta, TNFSF 1, TNFSF1, Tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1, Tumor necrosis factor superfamily member 1
Background: Tumor necrosis factor-beta(TNF-beta), previously called lymphotoxin-alpha(LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35 % identity and 50 % homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.
- ID gène
- 4049
- NCBI Accession
- NP_000586
- UniProt
- P01374
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
-