Chordin Kit ELISA
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- Antigène Voir toutes Chordin (CHRD) Kits ELISA
- Chordin (CHRD)
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 62.5 pg/mL - 4000 pg/mL
- Seuil minimal de détection
- 62.5 pg/mL
- Application
- ELISA
- N° du produit (Fournisseur)
- koa0845
- Fournisseur
- Rockland
- Fonction
- Mouse Chordin Sandwich ELISA Kit for Quantitative Detection
- Marque
- AccuSignal™
- Type d'échantillon
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificité
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Production: Natural and recombinant mouse Chordin. There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- < 10 pg/mL
- Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product CHRD Kit ELISA
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- Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Chordin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Chordin standard solution and each sample is measured in duplicate.
- Commentaires
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Standard: Expression system for standard: NSO, Immunogen sequence: T27-S948
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Date de péremption
- 12 months
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- Antigène Voir toutes Chordin (CHRD) Kits ELISA
- Chordin (CHRD)
- Autre désignation
- Chordin (CHRD Produits)
- Sujet
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Synonyms: Chordin, Chrd, CHRD_HUMAN, MGC133038, Shortgastrulation protein, SOG
Background: Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.
- ID gène
- 12667
- NCBI Accession
- NP_001264970
- UniProt
- Q9Z0E2
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