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Chordin Kit ELISA

Rockland koa0845 CHRD Reactivité: Souris Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
N° du produit ABIN6720247
N° du produit (Fournisseur): koa0845
  • Antigène Voir toutes Chordin (CHRD) Kits ELISA
    Chordin (CHRD)
    Reactivité
    • 4
    • 3
    • 2
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5 pg/mL - 4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    N° du produit (Fournisseur)
    koa0845
    Fournisseur
    Rockland
    Fonction
    Mouse Chordin Sandwich ELISA Kit for Quantitative Detection
    Marque
    AccuSignal™
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificité

    Production: Natural and recombinant mouse Chordin. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité
    < 10 pg/mL
    Ingrédients
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product CHRD Kit ELISA
  • Indications d'application
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Chordin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Chordin standard solution and each sample is measured in duplicate.
    Commentaires

    Standard: Expression system for standard: NSO, Immunogen sequence: T27-S948

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Date de péremption
    12 months
  • Antigène Voir toutes Chordin (CHRD) Kits ELISA
    Chordin (CHRD)
    Autre désignation
    Chordin (CHRD Produits)
    Sujet

    Synonyms: Chordin, Chrd, CHRD_HUMAN, MGC133038, Shortgastrulation protein, SOG

    Background: Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.

    ID gène
    12667
    NCBI Accession
    NP_001264970
    UniProt
    Q9Z0E2
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