ADAMTS4 Kit ELISA

N° du produit ABIN6720258

Détail du produit ADAMTS4 Kit ELISA

Antigène: ADAMTS4 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 4 (ADAMTS4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625 pg/mL - 40000 pg/mL

Seuil minimal de détection: 625 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 4, A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4, A disintegrin like and metalloprotease with thrombospondin type 1 motif 4, ADAM metallopeptidase with thrombospondin type 1 motif 4, ADAM TS 4, ADAM TS4, ADAM-TS 4, ADAM-TS4, ADAMTS2, ADAMTS4, ADAMTS4 protein, ADMP 1, ADMP-1, ADMP1, Aggrecanase1, ATS4_HUMAN, KIAA0688

Background: ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

Gene Name: ADAMTS4

Production: Natural and recombinant human ADAMTS4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: F213-C685

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human ADAMTS4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ADAMTS4 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 625pg/mL-40,000pg/mL

Other Performance Data: DOE: 2-8° C - 09/16/18 -20° C - 03/19/19

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: ADAMTS4 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 4 (ADAMTS4))

Autre désignation: ADAMTS4 (ADAMTS4 Produits)

Synonymes: 3830423K05 Kit ELISA, ADAM-TS4 Kit ELISA, ADAMTS-2 Kit ELISA, ADMP-1 Kit ELISA, mKIAA0688 Kit ELISA, ADAMTS-4 Kit ELISA, ADAMTS4 Kit ELISA, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif 4 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif, 4 Kit ELISA, Adamts4 Kit ELISA, ADAMTS4 Kit ELISA

ID gène: 9507

NCBI Accession: NP_001307265

UniProt: O75173