SFTPD Kit ELISA

N° du produit ABIN6720259

Détail du produit SFTPD Kit ELISA

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625 pg/mL - 40000 pg/mL

Seuil minimal de détection: 625 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: COLEC 7, COLEC7, Collectin-7, Collectin7, Lung surfactant protein D, PSP D, PSP-D, PSP-D Surfactant protein D, PSPD, Pulmonary surfactant apoprotein, Pulmonary surfactant associated protein D, Pulmonary surfactant associated protein PSP-D, Pulmonary surfactant-associated protein D, SFTP 4, SFTP4, SFTPD, SFTPD_HUMAN, SP D, SP-D, Surfactant associated protein pulmonary 4, Surfactant pulmonary associated protein D

Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Gene Name: SFTPD

Production: Natural and recombinant human SP-D. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A21-F419

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human SP-D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human SP-D standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 625pg/mL-40,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Autre désignation: SP-D (SFTPD Produits)

Synonymes: COLEC7 Kit ELISA, PSP-D Kit ELISA, SFTP4 Kit ELISA, SP-D Kit ELISA, BDE Kit ELISA, BDSD Kit ELISA, HOX4I Kit ELISA, SPD Kit ELISA, AI573415 Kit ELISA, Sftp4 Kit ELISA, surfactant protein D Kit ELISA, homeobox D13 Kit ELISA, surfactant associated protein D Kit ELISA, SFTPD Kit ELISA, HOXD13 Kit ELISA, Sftpd Kit ELISA, SP-D Kit ELISA

ID gène: 6441

NCBI Accession: NP_003010

UniProt: P35247