MME Kit ELISA

N° du produit ABIN6720269

Détail du produit MME Kit ELISA

Antigène: MME (Membrane Metallo-Endopeptidase (MME))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 93.7 pg/mL - 6000 pg/mL

Seuil minimal de détection: 93.7 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Atriopeptidase, CALLA, CD10, CD10 antigen, Common acute lymphocytic leukemia antigen, Enkephalinase, EPN, gp100, Membrane metallo endopeptidase, Membrane metallo endopeptidase variant 1, Membrane metallo endopeptidase variant 2, Membrane metalloendopeptidase, Membrane metalloendopeptidase neutral endopeptidase enkephalinase, Membrane metalloendopeptidase variant 1, Membrane metalloendopeptidase variant 2, NEP, NEP_HUMAN, Neprilysin, Neutral endopeptidase, SFE, Skin fibroblast elastase

Background: Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), CD10, and common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Gene Name: MME

Production: Natural and recombinant mouse CD10. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Y52-W750

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m mouse CD10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD10 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 93.7pg/mL-6000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: MME (Membrane Metallo-Endopeptidase (MME))

Autre désignation: Neprilysin - CD10 (MME Produits)

Synonymes: CALLA Kit ELISA, CD10 Kit ELISA, NEP Kit ELISA, SFE Kit ELISA, 6030454K05Rik Kit ELISA, C85356 Kit ELISA, Nep Kit ELISA, neprilysin Kit ELISA, membrane metalloendopeptidase Kit ELISA, membrane metallo endopeptidase Kit ELISA, membrane metallo-endopeptidase Kit ELISA, MME Kit ELISA, Mme Kit ELISA

Classe de substances: Chemical

ID gène: 17380

NCBI Accession: NP_001276391

UniProt: Q61391

Pathways: Signalisation RTK, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration