sL-Selectin Kit ELISA

N° du produit ABIN6720287

Détail du produit sL-Selectin Kit ELISA

Antigène: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625 pg/mL - 40000 pg/mL

Seuil minimal de détection: 625 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: A.11, AI528707, CD62 antigen ligand, CD62 antigen-like family member L, gp90 MEL, hLHRc, IgA nephropathy, susceptibility to, includedL Selectin, LAM1, LECAM1, Leu8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LSEL, Ly-22, Ly-m22, LYAM1, Lymph node homing receptor, Lymphocyte adhesion molecule 1, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, PLNHR, Selectin L, Selectin, lymphocyte, SELL, TQ1

Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Gene Name: SELL

Production: Natural and recombinant rat sL-Selectin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: W39-N332

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL,10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL rat L-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat L-Selectin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 625pg/mL-40000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Autre désignation: sL-Selectin (sL-Selectin Produits)

ID gène: 29259

NCBI Accession: NP_062050

UniProt: Q63762