LUM Kit ELISA
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- Antigène Voir toutes LUM Kits ELISA
- LUM (Lumican (LUM))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 156 pg/mL - 10000 pg/mL
- Seuil minimal de détection
- 156 pg/mL
- Application
- ELISA
- N° du produit (Fournisseur)
- koa0733
- Fournisseur
- Rockland
- Fonction
- Human Lumican Sandwich ELISA Kit for Quantitative Detection
- Marque
- AccuSignal™
- Type d'échantillon
- Cell Culture Supernatant, Plasma (heparin), Serum
- Analytical Method
- Quantitative
- Specificité
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Production: Natural and recombinant human Lumican. There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- < 10 pg/mL
- Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product LUM Kit ELISA
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- Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Lumican standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Lumican standard solution and each sample be measured in duplicate.
- Commentaires
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Standard: Expression system for standard: NSO, Immunogen sequence: Q19-N338
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Date de péremption
- 12 months
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- Antigène Voir toutes LUM Kits ELISA
- LUM (Lumican (LUM))
- Autre désignation
- Lumican (LUM Produits)
- Sujet
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Synonyms: Keratan sulfate proteoglycan lumican, KSPG Lumican, LDC, LUM, LUM_HUMAN, Lumican, Lumican precursor, SLRR2D
Background: Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene(SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.
- ID gène
- 4060
- NCBI Accession
- NP_002336
- UniProt
- P51884
- Pathways
- Glycosaminoglycan Metabolic Process
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