TXN Kit ELISA

N° du produit ABIN6720303

Détail du produit TXN Kit ELISA

Antigène: TXN (Thioredoxin (TXN))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: ADF, ATL-derived factor, DKFZp686B1993, MGC61975, SASP, Surface associated sulphydryl protein, Surface-associated sulphydryl protein, THIO_HUMAN, Thioredoxin, TRDX, TRX 1, Trx, TRX1, TXN, TXN protein

Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.

Gene Name: TXN

Production: Natural and recombinant human Thioredoxin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: V2-V105

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Thioredoxin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Thioredoxin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TXN (Thioredoxin (TXN))

Autre désignation: Thioredoxin (TXN Produits)

Synonymes: TRDX Kit ELISA, TRX Kit ELISA, TRX1 Kit ELISA, ADF Kit ELISA, AW550880 Kit ELISA, Trx1 Kit ELISA, Txn Kit ELISA, trx Kit ELISA, TXN Kit ELISA, SCM1.18 Kit ELISA, trxA3 Kit ELISA, thioredoxin Kit ELISA, TR-1 Kit ELISA, Trx-1 Kit ELISA, thioredoxin Kit ELISA, thioredoxin 1 Kit ELISA, thioredoxin L homeolog Kit ELISA, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) Kit ELISA, thioredoxin pseudogene Kit ELISA, cytosolic thioredoxin Trx1 Kit ELISA, TXN Kit ELISA, Txn1 Kit ELISA, txn.L Kit ELISA, txn Kit ELISA, trx Kit ELISA, SCO0885 Kit ELISA, PHG25ORF099c Kit ELISA, LOC100349046 Kit ELISA, Txn Kit ELISA, trx1 Kit ELISA

ID gène: 7295

NCBI Accession: NP_001231867

UniProt: P10599

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis