Granulin Kit ELISA

N° du produit ABIN6720319

Détail du produit Granulin Kit ELISA

Antigène: Granulin (GRN)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Acrogranin, CLN11, GEP, GP88, Granulin A, Granulin B, Granulin C, Granulin D, Granulin E, Granulin epithelin, Granulin F, Granulin G, Granulin-7, Granulins, GRN, GRN_HUMAN, PC cell derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, Progranulin

Background: Granulin, also known as Proepithelin(PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Gene Name: GRN

Production: Natural and recombinant human Progranulin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: T18-L593

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Progranulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or human milk to each empty well. We recommend that each human Progranulin standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 1.56 ng/mL-100 ng/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Granulin (GRN)

Autre désignation: Progranulin (GRN Produits)

Synonymes: GRN Kit ELISA, grn Kit ELISA, wu:fb11g12 Kit ELISA, wu:fj18f05 Kit ELISA, MGC53354 Kit ELISA, DKFZp459H066 Kit ELISA, CLN11 Kit ELISA, GEP Kit ELISA, GP88 Kit ELISA, PCDGF Kit ELISA, PEPI Kit ELISA, PGRN Kit ELISA, epithelin Kit ELISA, acrogranin Kit ELISA, granulin precursor Kit ELISA, granulin a Kit ELISA, Granulins Kit ELISA, granulin L homeolog Kit ELISA, granulin Kit ELISA, GRN Kit ELISA, grna Kit ELISA, grn Kit ELISA, grn.L Kit ELISA, granulin Kit ELISA, ORF1 granulin Kit ELISA, Grn Kit ELISA

Classe de substances: Viral Protein

ID gène: 2896

NCBI Accession: NP_002078

UniProt: P28799