TGFB3 Kit ELISA

N° du produit ABIN6720332

N° du produit (Fournisseur): koa0630

Détail du produit TGFB3 Kit ELISA

Antigène: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

N° du produit (Fournisseur): koa0630

Fournisseur: Rockland

Fonction: Rat TGF beta 3 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Serum, Urine

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant rat TGF-beta 3. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat TGF-beta 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated rat cell culture supernates, serum, plasma(EDTA) or urine to each empty well. It is recommended that each rat TGF-beta 3 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: sf21, Immunogen sequence: A301-S412

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Autre désignation: TGF-beta 3 (TGFB3 Produits)

Sujet:

Synonyms: ARVD, ARVD1, FLJ16571, MGC105479, MGC118722, prepro-transforming growth factor beta-3, RNHF, TGF beta 3, TGF beta3, TGF-beta-3, TGFB 3, TGFB3, TGFB3_HUMAN, transforming growth factor beta 3, Transforming growth factor beta-3

Background: Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.

ID gène: 25717

NCBI Accession: NP_037306

UniProt: Q07258

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus