TGFA Kit ELISA

N° du produit ABIN6720341

Détail du produit TGFA Kit ELISA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Milk, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: EGF like TGF, ETGF, OTTHUMP00000203300, OTTHUMP00000203301, OTTHUMP00000203345, OTTHUMP00000204192, Protransforming growth factor alpha, RATTGFAA, TFGA, TGF A, TGF type 1, TGFA, Transforming growth factor alpha, Transforming growth factor alpha precursor, Wa1, Waved 1

Background: Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2,8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells, the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Gene Name: TGFA

Production: Natural and recombinant human TGF alpha. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: V40-A89

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TGF alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or milk to each empty well. It is recommended that each human TGF alpha standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF alpha (TGFA Produits)

Synonymes: TFGA Kit ELISA, LOC100009482 Kit ELISA, TGF alpha Kit ELISA, TGFA Kit ELISA, wa-1 Kit ELISA, wa1 Kit ELISA, RATTGFAA Kit ELISA, TGFAA Kit ELISA, NV15606 Kit ELISA, si:ch211-10e8.7 Kit ELISA, transforming growth factor alpha Kit ELISA, transforming growth factor alpha L homeolog Kit ELISA, transforming growth factor-alpha Kit ELISA, transforming growth factor, alpha Kit ELISA, TGFA Kit ELISA, TGF-A Kit ELISA, Tgfa Kit ELISA, tgfa.L Kit ELISA, LOC100121300 Kit ELISA, LOC100009150 Kit ELISA, tgfa Kit ELISA

ID gène: 7039

NCBI Accession: NP_001093161

UniProt: P01135

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway