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PRSS8 Kit ELISA

PRSS8 Reactivité: Souris Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
N° du produit ABIN6720348
  • Antigène Voir toutes PRSS8 Kits ELISA
    PRSS8 (Protease, serine, 8 (PRSS8))
    Reactivité
    • 9
    • 5
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.78 ng/mL - 50 ng/mL
    Seuil minimal de détection
    0.78 ng/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: 2410039E18Rik, AI313909, C79772, CAP1, Channel Activating Protease 1, mCAP1, Prostasin, Prostasin heavy chain, protease, serine, 8, Prss8, PRSS8_HUMAN, Serine protease 9

    Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60 % to 80 % increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

    Gene Name: PRSS8

    Production: Natural and recombinant mouse Prostasin. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: A30-Q289

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  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Prostasin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. We recommend that each mouse Prostasin standard solution and each sample is measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 0.78 ng/mL-50 ng/mL

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène Voir toutes PRSS8 Kits ELISA
    PRSS8 (Protease, serine, 8 (PRSS8))
    Autre désignation
    Prostasin (PRSS8 Produits)
    Synonymes
    CAP1 Kit ELISA, PROSTASIN Kit ELISA, prostasin Kit ELISA, 2410039E18Rik Kit ELISA, AI313909 Kit ELISA, C79772 Kit ELISA, fr Kit ELISA, mCAP1 Kit ELISA, protease, serine 8 Kit ELISA, protease, serine, 8 Kit ELISA, protease, serine 8 (prostasin) Kit ELISA, PRSS8 Kit ELISA, Prss8 Kit ELISA
    ID gène
    76560
    NCBI Accession
    NP_579929
    UniProt
    Q9ESD1
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