PRSS8 Kit ELISA
-
- Antigène Voir toutes PRSS8 Kits ELISA
- PRSS8 (Protease, serine, 8 (PRSS8))
-
Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.78 ng/mL - 50 ng/mL
- Seuil minimal de détection
- 0.78 ng/mL
- Application
- ELISA
- Fonction
- Sandwich ELISA for Quantitative Detection of Antigen
- Type d'échantillon
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
- Analytical Method
- Quantitative
- Attributs du produit
-
Synonyms: 2410039E18Rik, AI313909, C79772, CAP1, Channel Activating Protease 1, mCAP1, Prostasin, Prostasin heavy chain, protease, serine, 8, Prss8, PRSS8_HUMAN, Serine protease 9
Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60 % to 80 % increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.
Gene Name: PRSS8
Production: Natural and recombinant mouse Prostasin. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: A30-Q289
- Featured
- Discover our best selling PRSS8 Kit ELISA
- Top Product
- Discover our top product PRSS8 Kit ELISA
-
-
- Indications d'application
-
Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Prostasin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. We recommend that each mouse Prostasin standard solution and each sample is measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 0.78 ng/mL-50 ng/mL
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
-
- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- Antigène Voir toutes PRSS8 Kits ELISA
- PRSS8 (Protease, serine, 8 (PRSS8))
- Autre désignation
- Prostasin (PRSS8 Produits)
- Synonymes
- CAP1 Kit ELISA, PROSTASIN Kit ELISA, prostasin Kit ELISA, 2410039E18Rik Kit ELISA, AI313909 Kit ELISA, C79772 Kit ELISA, fr Kit ELISA, mCAP1 Kit ELISA, protease, serine 8 Kit ELISA, protease, serine, 8 Kit ELISA, protease, serine 8 (prostasin) Kit ELISA, PRSS8 Kit ELISA, Prss8 Kit ELISA
- ID gène
- 76560
- NCBI Accession
- NP_579929
- UniProt
- Q9ESD1
-