Angiopoietin 2 Kit ELISA

N° du produit ABIN6720360

Détail du produit Angiopoietin 2 Kit ELISA

Antigène: Angiopoietin 2 (ANGPT2)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: AGPT2, ANG 2, ANG-2, ANG2, Angiopoietin 2a, Angiopoietin 2B, Angiopoietin-2, Angiopoietin2, ANGP2_HUMAN, ANGPT 2, Angpt2, Tie2 ligand

Background: Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60 % homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Gene Name: ANGPT2

Production: Natural and recombinant human Angiopoietin-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Y19-F496

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. It is recommended that each human Angiopoietin-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Angiopoietin 2 (ANGPT2)

Autre désignation: Angiopoietin-2 (ANGPT2 Produits)

Synonymes: AGPT2 Kit ELISA, ANG2 Kit ELISA, Agpt2 Kit ELISA, Ang-2 Kit ELISA, Ang2 Kit ELISA, ang2 Kit ELISA, angpt2 Kit ELISA, ANGIOPOIETIN-2 Kit ELISA, ANG-2 Kit ELISA, ANGPT2 Kit ELISA, Angrp Kit ELISA, Rnase5b Kit ELISA, ANG3 Kit ELISA, C11orf2 Kit ELISA, C11orf3 Kit ELISA, FFR Kit ELISA, angiopoietin 2 Kit ELISA, angiopoietin 2b Kit ELISA, angiogenin, ribonuclease A family, member 2 Kit ELISA, VPS51, GARP complex subunit Kit ELISA, ANGPT2 Kit ELISA, Angpt2 Kit ELISA, angpt2b Kit ELISA, Ang2 Kit ELISA, VPS51 Kit ELISA

ID gène: 285

NCBI Accession: NP_001112359

UniProt: O15123

Pathways: Signalisation RTK