MMP2 Kit ELISA
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- Antigène Voir toutes MMP2 Kits ELISA
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 156 pg/mL - 10000 pg/mL
- Seuil minimal de détection
- 156 pg/mL
- Application
- ELISA
- Fonction
- Sandwich ELISA for Quantitative Detection of Antigen
- Type d'échantillon
- Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
- Analytical Method
- Quantitative
- Attributs du produit
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Synonyms: 72 kDa gelatinase, 72 kDa type IV collagenase, CLG 4, CLG 4A, CLG4, CLG4A, Collagenase type IV A, EC 3.4.24.24, Gelatinase A, Gelatinase alpha, Gelatinase neutrophil, Matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase), Matrix metalloproteinase 2, Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase), Matrix metalloproteinase II, Matrix metalloproteinase-2, MMP 2, MMP II, MMP-2, MMP2, MMP2_HUMAN, MONA, Neutrophil gelatinase, PEX, TBE 1, TBE-1
Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant human MMP-2, consisting of 631 amino acids with the molecular mass of 71KDa. The detected MMP-2 includes zymogen and active enzyme.
Gene Name: MMP2
Production: Natural and recombinant human total MMP-2. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: A30-C660
- Top Product
- Discover our top product MMP2 Kit ELISA
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- Indications d'application
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin), saliva or urine) to each empty well. It is recommended that each human MMP-2 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10000pg/mL
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Antigène Voir toutes MMP2 Kits ELISA
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
- Autre désignation
- MMP-2 (MMP2 Produits)
- Synonymes
- 2-MMP Kit ELISA, CG1794 Kit ELISA, DM2-MMP Kit ELISA, Dm2-MMP Kit ELISA, Dmel\\CG1794 Kit ELISA, MMP2 Kit ELISA, anon-WO0118547.84 Kit ELISA, dm-2MMP Kit ELISA, dmmp2 Kit ELISA, l(2)02353 Kit ELISA, mmp2 Kit ELISA, wu:fa99h12 Kit ELISA, wu:fk89d01 Kit ELISA, fgmmp-2 Kit ELISA, Mmp-2 Kit ELISA, LOC100135793 Kit ELISA, Clg4a Kit ELISA, GelA Kit ELISA, MMP-2 Kit ELISA, CLG4 Kit ELISA, CLG4A Kit ELISA, MMP-II Kit ELISA, MONA Kit ELISA, TBE-1 Kit ELISA, matrix metallopeptidase 2 Kit ELISA, Matrix metalloproteinase 2 Kit ELISA, matrix metalloproteinase 2 Kit ELISA, matrix metalloproteinase-2 Kit ELISA, MMP2 Kit ELISA, Mmp2 Kit ELISA, mmp2 Kit ELISA, LOC657982 Kit ELISA, LOC100135793 Kit ELISA
- ID gène
- 4313
- NCBI Accession
- NP_001121363
- UniProt
- P08253
- Pathways
- Activation of Innate immune Response
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