FURIN Kit ELISA

N° du produit ABIN6720393

Détail du produit FURIN Kit ELISA

Antigène: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Dibasic processing enzyme, Dibasic-processing enzyme, FES upstream region, FUR, FURIN, Furin membrane associated receptor protein, FURIN_HUMAN, PACE, Paired basic amino acid residue cleaving enzyme, Paired basic amino acid residue-cleaving enzyme, PCSK3, Proprotein convertase subtilisin/kexin type 3, SPC1

Background: Furin(Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance

Gene Name: FURIN

Production: Natural and recombinant human Furin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D108-E715

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Furin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or cell lysates to each empty well. It is recommended that each human Furin standard solution and each sample be measured in duplicate.

ELISA Dilution: 312pg/mL-20000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Autre désignation: Furin (FURIN Produits)

Synonymes: FURIN Kit ELISA, furin Kit ELISA, si:dkey-281a24.5 Kit ELISA, FUR Kit ELISA, LOC100220259 Kit ELISA, PACE Kit ELISA, spc1 Kit ELISA, xfurin Kit ELISA, MGC82710 Kit ELISA, PCSK3 Kit ELISA, SPC1 Kit ELISA, 9130404I01Rik Kit ELISA, Fur Kit ELISA, Pcsk3 Kit ELISA, Pace Kit ELISA, furin, paired basic amino acid cleaving enzyme Kit ELISA, furin (paired basic amino acid cleaving enzyme) a Kit ELISA, furin (paired basic amino acid cleaving enzyme) b Kit ELISA, furin, paired basic amino acid cleaving enzyme L homeolog Kit ELISA, furin (paired basic amino acid cleaving enzyme) Kit ELISA, FURIN Kit ELISA, furina Kit ELISA, furinb Kit ELISA, furin Kit ELISA, furin.L Kit ELISA, Furin Kit ELISA

ID gène: 5045

NCBI Accession: NP_001276752

UniProt: P09958

Pathways: Signalisation Notch, Neurotrophin Signaling Pathway